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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Construction and expression of SpOMT2884 in E. coli . ( A ) Map of the expression vector pETDuet-SpOMT2884; ( B ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-SpOMT2884. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 8 h induction (lane 3) were separated with SDS-PAGE. Arrows indicate the expressed SpOMT2884. M represents the molecular weight markers.
Article Snippet: The
Techniques: Expressing, Plasmid Preparation, Polyacrylamide Gel Electrophoresis, SDS Page, Recombinant, Lysis, Molecular Weight
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Ultra-performance liquid chromatography (UPLC) profiles of 8-hydroxydaidzein standard ( A ) and fermentation broth (( B ): 0 h induction; ( C ): 24 h induction) of recombinant E. coli expressing SpOMT2884. The recombinant strain was cultivated at 37 °C in shacking flasks with LeMaster and Richards minimal medium (LR medium) containing 100 μM 8-hydroxydaidzein. After induction with 0.5 mM isopropyl-β- d -thiogalactopyranoside (IPTG), aliquot samples of the fermentation were collected and analyzed with UPLC. The detailed protocols for fermentation and UPLC are described in Materials and Methods.
Article Snippet: The
Techniques: Liquid Chromatography, Recombinant, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Production of Two Novel Methoxy-Isoflavones from Biotransformation of 8-Hydroxydaidzein by Recombinant Escherichia coli Expressing O -Methyltransferase SpOMT2884 from Streptomyces peucetius
doi: 10.3390/ijms161126070
Figure Lengend Snippet: Diagram of the biotransformation of 8-hydroxydaidzein by the recombinant E. coli expressing SpOMT2884.
Article Snippet: The
Techniques: Recombinant, Expressing
Journal: Journal of Cancer
Article Title: A Model to Predict Prognosis of Renal Cell Clear Cell Carcinoma Based on 3 Angiogenesis-related Long Non-coding RNAs
doi: 10.7150/jca.94685
Figure Lengend Snippet: Identification of AR-lncRNAs. (A-D) Heatmaps and volcano plots showing the differentially expressed lncRNAs (A, B) or genes (C, D) between ccRCC tissues and paracancerous tissues in the TCGA. In the heatmap, the red parts represent upregulated lncRNAs/genes, and the blue parts represent downregulated lncRNAs/genes. In the volcano plot, the green dots represent downregulated lncRNAs/genes, the red dots represent upregulated lncRNAs/genes, and the black dots represent lncRNAs/genes with no differential expression (log2 |FC| > 1, p < 0.05). (E) Venn diagram illustrating 39 angiogenesis-related genes identified from the TCGA, HALLMARK and GSEA databases. (F) Forest plot showing the 9 prognostic differentially expressed sAR-lncRNAs according to univariate COX regression analysis. (G) LASSO regression analysis was carried out to identify 5 sAR-lncRNAs. (H) The optimal LASSO model was constructed with the best parameter (λ=0.03). (I) Forest plot showing the 3 prognostic differentially expressed sAR-lncRNAs according to multivariate COX regression analysis. AR-lncRNAs, angiogenesis-related lncRNAs; ccRCC, clear cell renal cell carcinoma; TCGA, The Cancer Genome Atlas; sAR-lncRNAs, survival AR-lncRNAs.
Article Snippet: Angiogenesis-related genes (ARGs) were extracted from The Molecular Signatures Database gene sets ANGIOGENESIS-M14493 and HALLMARK_
Techniques: Quantitative Proteomics, Construct